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Laboratory Protocols for Download.
NEW. Updated 8 December, 2021. This page will be updated frequently
with protocols optimized for use with the GeneDetect® range of products
for radioactive and non-radioactive in situ hybridization.
Please download protocols below.
NEW. Use Google scholar to search for in situ hybridization protocol papers to
help you get your in situ hybridization experiments working well. For
example, search for earlier published articles using GeneDetect probes by searching for
GeneDetect or search for in situ hybridization optimization to find
optimized in situ hybridization protocols. Of course, please also contact us
directly for direct technical support. Google scholar is a great resource. Use
it!!
NEW. Note you can currently find > 700 recent
publications using GeneDetect products by typing GeneDetect into the link
below.
GeneDetect® oligonucleotide
probes.
| 1. Handling and storage
instructions. |
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In situ hybridization protocols.
| FROZEN SECTIONS |
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| 1. 35S-labeled
GeneDetect® probe, Detection by emulsion or film autoradiography. |
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| 2. GreenStar* FITC-hyperlabeled
probe, Detection by direct fluorescence, AP (alkaline phosphatase) or
tyramide signal amplification (TSA). |
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| 3. GreenStar* DIG-hyperlabeled
probe, Detection by direct fluorescence, AP (alkaline phosphatase) or
tyramide signal amplification (TSA). |
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| 4. GreenStar* BIOTIN-hyperlabeled
probe, Detection by AP (alkaline phosphatase) or HRP (Horseradish
Peroxidase) with or without tyramide
signal amplification (TSA).
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| PARAFFIN SECTIONS |
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| 1. GreenStar* BIOTIN-hyperlabeled probe, Detection by AP (alkaline
phosphatase) or HRP (Horseradish Peroxidase) with or without tyramide
signal amplification (TSA). |
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| 2. GreenStar* DIG-hyperlabeled probe, Detection by
direct fluorescence, AP (alkaline phosphatase) or tyramide
signal amplification (TSA). |
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| 3. DIG-labeled GeneDetect® probe, Detection by AP (alkaline
phosphatase), FITC or rhodamine. |
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Use the FREE Acrobat Reader available from the Adobe website to read
PDF files. Are you having problems reading the .pdf files? Click here.
GeneDetect®
is a registered trademark of GeneDetect.com Limited
(USPTO Reg 2765935).
The
use of oligonucleotide probes for localizing mRNA in tissue sections is licensed
from Syngene (Australia). The Syngene "In situ Hybridization"
patents licensed include US Patents 5,597,692 and 6,265,156, European Patent
0,175,776, Canadian Patent 1,251,119, Australian Patent 579,631 and Japanese
Patent 1,970,487.
§
The use of digoxigenin technology to label nucleic acids is licensed under
patents (EP 0 324 474, US 5.344.757, US 5.702.888, US 5.354.657, JP 1999884 and
HK 1169) owned by Roche Diagnostics GmbH. For further information on products to
label and detect digoxigenin-modified nucleic acids refer to
www.roche-applied-science.com/dig/
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